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Using our culture protocol, we have analyzed the development of late stage egg chambers using Green Fluorescent Protein fused to the actin-binding protein Moesin (Bloor and Kiehart 2001) or to histone 2A (Clarkson and Saint 1999). These studies reveal the dynamic events of tube formation and elongation, and demonstrate that the epithelial sheet folds as a single layer into a tube. Fixed tissue stained for roof- and floor-cell markers compliments the live image analysis.